- Finish rinsing and filtering prepared microspheres with hexanes.
- Continue microsphere preparation.
- Add dye to prepared microspheres in preparation for diffusion testing on microspheres with dye additive.
Microsphere Dye Addition
- The general protocol for dye addition to PVA hydrogel/microspheres described on 2013/02/08 was followed.
- An internal concentration of 1μM was wanted for the prepared microspheres and hydrogels
- Dye was added to all the microspheres prepared on 2013/02/20
1μM Rhodamine 6G Dye Concentration (90:10)
M1V1 = M2V2
1μM (RG6)x 10mL = (92μM)V2 V2 = 109μL
1μM Rhodamine 6G Dye Concentration (50:50)
M1V1 = M2V2
1μM (RG6)x 10mL = (165μM)V2 V2 = 61μL
- The general protocol for microsphere preparation described on 2013/02/20 and 2013/02/22.
- The following preparations were run on a 0.1g scale as opposed to a 1g scale in the hopes of preventing the formation of hydrogels.
- A very small amount of PVA/clay was added to each vial and the vial was filled with safflower oil prior to the rapid freezing in order to achieve a true emulsion of microspheres in the organic safflower oil. This may prevent the formation of hydrogels.
|PVOH vs. Clay Ratio
||PVOH 146K Mass (g)
||Actual Clay Mass (g)
||H2O Added (mL)
||Safflower oil Added (mL)
| 50:50||110% CEC NaMT||0.05010||0.05100||20||35
| 90:10||110% CEC NaMT||0.01070||0.09100||20||35
- Dye was added to the microspheres prepared on 2013/02/20 prior to a DSC analysis. These samples will need to be reprepared for DSC analysis.