User:Nkuldell:M13 redesign: Difference between revisions
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===Life Cycle of the Phage=== | ===Life Cycle of the Phage=== | ||
===M13 phage proteins=== | ===M13 phage proteins=== | ||
[[Image:Macintosh HD-Users-nkuldell-Desktop-M13K07 plasmid-phageproteins.jpeg|thumb|left| phage proteins]] | |||
NCBI link to seq: [[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=56713234]] <br> | NCBI link to seq: [[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=56713234]] <br> | ||
Source: Phage Display of Peptides and Proteins: a laboratory manual. Kay, Winter, McCafferty. Ch 1. [[http://www.amazon.com/Phage-Display-Peptides-Proteins-Laboratory/dp/0124023800]]<br> | Source: Phage Display of Peptides and Proteins: a laboratory manual. Kay, Winter, McCafferty. Ch 1. [[http://www.amazon.com/Phage-Display-Peptides-Proteins-Laboratory/dp/0124023800]]<br> |
Revision as of 10:49, 26 October 2006
Due Diligence
Life Cycle of the Phage
M13 phage proteins
NCBI link to seq: [[1]]
Source: Phage Display of Peptides and Proteins: a laboratory manual. Kay, Winter, McCafferty. Ch 1. [[2]]
protein | aa (mature) | function | notes |
---|---|---|---|
I | 348 | assembly | includes gXI within, the two form structure in cytoplasmic membrane for phage maturation |
II | 410 | replication of DNA + strand | includes gX within |
III | 406 | phage tail protein (5 copies) | tag at N-term |
IV | 405 | assembly | organizes to pore in OM |
V | 87 | binds ssDNA | recognizes DNA PS |
VI | 112 | phage tail protein (5 copies) | packages with pIII |
VII | 33 | phage head protein (5 copies) | packages with p9 |
VIII | 50 | phage coat protein (2700 copies) | seg seq cleaved, matured form at aa 23 = clones to N-term, "Petrenko" site [[3]] |
IX | 32 | phage head protein (5 copies) | stop codon overlaps start of gVIII |
X | 111 | DNA replication | internal reinitiation inside gII |
XI | 108 | assembly | translation reinitiation inside gI |