User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2014/08/27: Difference between revisions
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Next, solutions were made with gold:protein ratios of 10:1 to 60:1. (See the table). The test tubes were placed in at 80°C for three hours, starting at 13:00. | Next, solutions were made with gold:protein ratios of 10:1 to 60:1. (See the table). The test tubes were placed in at 80°C for three hours, starting at 13:00. | ||
{| class="wikitable" | |||
|- | |||
! Au | |||
! Lysozyme | |||
{| | ! H<sub>2</sub>O | ||
| | |- | ||
| 2.5 mL | |||
| 2.5 mL | |||
| 0 mL | |||
|- | |- | ||
| 2.5 mL | |||
| 1.25 mL | |||
| 1.25 mL | |||
|2.5 mL | |||
|1.25 mL | |||
|1.25 mL | |||
|- | |- | ||
|2.5 mL | | 2.5 mL | ||
|0.835 mL | | 0.835 mL | ||
|1. | | 1.635 mL | ||
|- | |- | ||
|2.5 mL | | 2.5 mL | ||
|0.625 mL | | 0.625 mL | ||
|1.875 mL | | 1.875 mL | ||
|- | |- | ||
|2.5 mL | | 2.5 mL | ||
|0.5 mL | | 0.5 mL | ||
|2 mL | | 2 mL | ||
|- | |- | ||
|2.5 mL | | 2.5 mL | ||
|0.415 mL | | 0.415 mL | ||
|2.08 mL | | 2.08 mL | ||
|- | |- | ||
|} | |||
< | ==Kinetics== | ||
0.5 mL of HAuCl<sub>4</sub> stock and 0.166 mL of Lysozyme stock were added to three test tubes for a 30:1 gold to protein ratio. 4.33 mL of 80°C water was added. Kinetics runs were done every 30 minutes with 2.5 mL of sample in plastic cuvette from 800-360 nm. Started at 13:35. | |||
Revision as of 11:21, 2 September 2014
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Making HAuCl4 and Lysozyme Stocks
Next, solutions were made with gold:protein ratios of 10:1 to 60:1. (See the table). The test tubes were placed in at 80°C for three hours, starting at 13:00.
Kinetics0.5 mL of HAuCl4 stock and 0.166 mL of Lysozyme stock were added to three test tubes for a 30:1 gold to protein ratio. 4.33 mL of 80°C water was added. Kinetics runs were done every 30 minutes with 2.5 mL of sample in plastic cuvette from 800-360 nm. Started at 13:35.
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