User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2014/09/24: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 9: Line 9:


A bradford analysis was run of the dialysis solutions and of the protein/colloid solutions. For the dialysis solutions, 600 µL of each solution, 200 µL of bradford dye, and 100 µL of 50 mM Tris/NaCl buffer were mixed. For the dialyzed protein/colloid, 100 µL of protein/colloid, 700 µL of tris buffer, and 200 µL of bradford dye.
A bradford analysis was run of the dialysis solutions and of the protein/colloid solutions. For the dialysis solutions, 600 µL of each solution, 200 µL of bradford dye, and 100 µL of 50 mM Tris/NaCl buffer were mixed. For the dialyzed protein/colloid, 100 µL of protein/colloid, 700 µL of tris buffer, and 200 µL of bradford dye.
'''Results'''
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Solution'''
| align="center" style="background:#f0f0f0;"|'''Conc of Lysozyme (µg/mL)'''
| align="center" style="background:#f0f0f0;"|'''Abs'''
|-
| 3500 NaCl Soak||-3.344660194||0.028
|-
| 3500 H2O Soak||-3.975728155||0.015
|-
| 3500 Gly Soak||2.529126214||0.149
|-
| 25k Gly Soak||-0.09223301||0.095
|-
| 3500 Gly Colloid||1.703883495||0.132
|-
| 3500 NaCl/Lys Bag||17.91747573||0.466
|-
| 3500 H2O/ Lys Bag||18.54854369||0.479
|-
| 3500 Gly/Lys Bag||20.05339806||0.51
|-
| 3500 Gly/Coll Bag||2.82038835||0.155
|}
[[Image:Bradford_Dialysis_Analysis.png|500px]]




Revision as of 11:03, 24 September 2014

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Bradford Analysis of Dialyzed Solutions

A bradford analysis was run of the dialysis solutions and of the protein/colloid solutions. For the dialysis solutions, 600 µL of each solution, 200 µL of bradford dye, and 100 µL of 50 mM Tris/NaCl buffer were mixed. For the dialyzed protein/colloid, 100 µL of protein/colloid, 700 µL of tris buffer, and 200 µL of bradford dye.

Results

Solution Conc of Lysozyme (µg/mL) Abs
3500 NaCl Soak -3.344660194 0.028
3500 H2O Soak -3.975728155 0.015
3500 Gly Soak 2.529126214 0.149
25k Gly Soak -0.09223301 0.095
3500 Gly Colloid 1.703883495 0.132
3500 NaCl/Lys Bag 17.91747573 0.466
3500 H2O/ Lys Bag 18.54854369 0.479
3500 Gly/Lys Bag 20.05339806 0.51
3500 Gly/Coll Bag 2.82038835 0.155



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Paul_Rothenberg/Notebook/CHEM_571_Fall_2014/2014/09/24&oldid=823161"