User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2014/10/14: Difference between revisions
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Bradford, UV-VIS, and Fluorescence analysis were run on the dialysis samples from the KI dialysis. 1 in 4 Bradford solution was prepared and the samples were prepped the same method as previously. A blank was also run. For UV-VIS and fluorescence, a 1 in 100 dilution of each was prepared and run. UV-VIS was run from 200-400 nm. | Bradford, UV-VIS, and Fluorescence analysis were run on the dialysis samples from the KI dialysis. 1 in 4 Bradford solution was prepared and the samples were prepped the same method as previously. A blank was also run. For UV-VIS and fluorescence, a 1 in 100 dilution of each was prepared and run. UV-VIS was run from 200-400 nm. | ||
'''Bradford Results''' | |||
[[Image:Bradford_Lysozyme_KI_10_14_MGPR.png|500px]] | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Wavelength''' | |||
| align="center" style="background:#f0f0f0;"|'''577''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration (ug/mL)''' | |||
|- | |||
| Stock||0.121||1.169902913 | |||
|- | |||
| 2 mM||0.115||0.878640777 | |||
|- | |||
| 5 mK||0.124||1.315533981 | |||
|- | |||
| 10 mM||0.114||0.830097087 | |||
|- | |||
| 25 mM||0.111||0.684466019 | |||
|- | |||
| 50 mM||0.115||0.878640777 | |||
|- | |||
| | |||
|} | |||
Revision as of 09:16, 24 October 2014
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SDS-PAGE PrepSDS-PAGE was prepared according to the following:
Dialysis AnalysisBradford, UV-VIS, and Fluorescence analysis were run on the dialysis samples from the KI dialysis. 1 in 4 Bradford solution was prepared and the samples were prepped the same method as previously. A blank was also run. For UV-VIS and fluorescence, a 1 in 100 dilution of each was prepared and run. UV-VIS was run from 200-400 nm.
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