User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/02/03: Difference between revisions
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'''Solutions Tested''' | '''Solutions Tested''' | ||
#Pepsin | #<u>Pepsin</u> | ||
## | ##57.6 µM (2.5 mL buffer, 2.5 mL enzyme) | ||
## | ##11.56 µM (4.5 mL buffer, 0.5 mL enzyme) | ||
## | ##1.156 µM (4.95 mL buffer, 0.05 mL enzyme) | ||
## | ##0.12 µM (5 mL buffer, 0.005 mL enzyme) | ||
#Trypsin | #<u>Trypsin</u> | ||
## | ##57.6 µM (2.5 mL buffer, 2.5 mL enzyme) | ||
## | ##11.56 µM (4.5 mL buffer, 0.5 mL enzyme) | ||
## | ##1.156 µM (4.95 mL buffer, 0.05 mL enzyme) | ||
## | ##0.12 µM (5 mL buffer, 0.005 mL enzyme) | ||
#Chymotrypsin | #<u>Chymotrypsin</u> | ||
## | ##57.6 µM (2.5 mL buffer, 2.5 mL enzyme) | ||
## | ##11.56 µM (4.5 mL buffer, 0.5 mL enzyme) | ||
## | ##1.156 µM (4.95 mL buffer, 0.05 mL enzyme) | ||
## | ##0.12 µM (5 mL buffer, 0.005 mL enzyme) | ||
#Proteinase K (K) | #<u>Proteinase K (K)</u> | ||
## | ##57.6 µM (2.5 mL buffer, 2.5 mL enzyme) | ||
## | ##11.56 µM (4.5 mL buffer, 0.5 mL enzyme) | ||
## | ##1.156 µM (4.95 mL buffer, 0.05 mL enzyme) | ||
## | ##0.12 µM (5 mL buffer, 0.005 mL enzyme) | ||
NB - Pepsin has an optimal pH of 1.5-2, so a pH 2.14, 5 mM Citrate, 0.2 M NaCl buffer was made for Pepsin use. That may affect the results. | |||
'''Reaction Conditions''' | |||
The AuNP fibers were spun down at 300 RPM for 4 minutes. The supernatant was removed, and the prepared solutions were added on top of the solid fibers. The tubes were placed in a shaker at 37°C. They will be analyzed tomorrow. | |||
==Results== | ==Results== |
Revision as of 17:27, 3 February 2015
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Qualitative Analysis of Protease ActivityObjectiveContinue the qualitative analysis of protease activity on AuNP fibers. Pepsin, Trypsin, Chymotrypsin, and Proteinase K will be tested. ProcedureStock Solutions
Solutions Tested
NB - Pepsin has an optimal pH of 1.5-2, so a pH 2.14, 5 mM Citrate, 0.2 M NaCl buffer was made for Pepsin use. That may affect the results. Reaction Conditions The AuNP fibers were spun down at 300 RPM for 4 minutes. The supernatant was removed, and the prepared solutions were added on top of the solid fibers. The tubes were placed in a shaker at 37°C. They will be analyzed tomorrow. Results |