User:Pauline Wonnenberg/Notebook/CHEM 471 Fall 2017/2017/08/30: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Objectives== | ||
*Remake AuNP Solutions | |||
*Prepare Amino Acid Solutions | |||
*Prepare AuNP using Amino Acid Solutions | |||
*Measure UV & Fluorescence of AA Stock Solutions | |||
==Description== | |||
* '''AuNP not formed during previous laboratory; trial 2 for solutions below:''' | * '''AuNP not formed during previous laboratory; trial 2 for solutions below:''' | ||
Power of ten error was found, see updated calculations below: | |||
;Goal: 2.5mM HAuCl<sub>4</sub> into 50mL flask | |||
;Calculations: 0.05L x 2.5x10<sup>-3</sup>M = 1.25x10<sup>-4</sup>moles | |||
:HAuCl<sub>4</sub> = 339.785g/mol | |||
:339.785g/mol : 1.25x10<sup>-4</sup>moles = 0.04247g = 42.47mg of HAuCl<sub>4</sub> | |||
<br> | <br> | ||
* '''Preparation of 3 different amino acid solutions with the same concentration as BSA (250 μM):''' | * '''Preparation of 3 different amino acid solutions with the same concentration as BSA (250 μM):''' | ||
;Choice of amino acids Allopathic - Isoleucine; Aromatic - Phenylalanine; Amine - Arginine | |||
:Isoleucine Calculations: 0.05L x 250x10<sup>-6</sup>M = 1.25x10<sup>-5</sup>moles | |||
::Molar mass of Isoleucine: 131.18g/mol | |||
::131.18g/mol x 1.25x10<sup>-5</sup>M = 0.001639grams | |||
:Phenylalanine Calculations: 0.05L x 250x10<sup>-6</sup>M = 1.25x10<sup>-5</sup>moles | |||
::Molar mass of Phenylalanine: 165.19g/mol | |||
::165.19g/mol x 1.25x10<sup>-5</sup>M = 0.002065grams | |||
:Arginine Calculations: 0.05L x 250x10<sup>-6</sup>M = 1.25x10<sup>-5</sup>moles | |||
::Molar mass of Arginine: 174.20g/mol | |||
::174.20g/mol x 1.25x10<sup>-5</sup>M = 0.0021775grams | |||
<br> | <br> | ||
==Data== | |||
* '''Prepare AuNP using Amino Acid Solutions:''' | |||
<table class="demo"> | <table class="demo"> | ||
<caption>Aromatic - Phe</caption> | <caption>Aromatic - Phe</caption> | ||
| Line 45: | Line 51: | ||
<td>3.244 </td> | <td>3.244 </td> | ||
</tr> | </tr> | ||
</table> | </table><br> | ||
<table class="demo"> | <table class="demo"> | ||
<caption>Amine - Arg</caption> | <caption>Amine - Arg</caption> | ||
| Line 65: | Line 70: | ||
<td>3.272 </td> | <td>3.272 </td> | ||
</tr> | </tr> | ||
</table> | </table><br> | ||
<table class="demo"> | <table class="demo"> | ||
<caption>Aliphatic - Iso</caption> | <caption>Aliphatic - Iso</caption> | ||
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<td>3.338 </td> | <td>3.338 </td> | ||
</tr> | </tr> | ||
</table> | </table><br> | ||
<br>Following the above table, here is the UV-Vis spectrometer collected analyzing the | *'''Measure UV of AA Stock solutions:''' | ||
Following the above table, here is the UV-Vis spectrometer collected analyzing a sample of the Phenylalanine stock.<br>[[Image:08-30_Phenylalanine.png|400px]]<br> | |||
<br>Following the above table, here is the UV-Vis spectrometer collected analyzing a sample of the Arginine stock.<br>[[Image:08-30_Amine_Graph.png|400px]]<br> | |||
<br>Following the above table, here is the UV-Vis spectrometer collected analyzing a sample of the Isoleucine stock.<br>[[Image:08-30_Isoleucine_Graph.png|400px]]<br> | |||
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Latest revision as of 12:57, 21 November 2017
 Project name
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 Main project page Previous entry Next entry
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Objectives
Description
Power of ten error was found, see updated calculations below:
Data
Following the above table, here is the UV-Vis spectrometer collected analyzing a sample of the Phenylalanine stock.
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