User:Puja Mody/Notebook/Chem 571: Gold Nanoparticles/2012/09/11: Difference between revisions

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* Run UV-Vis on AuNP/BSA samples 120, 128, 130, 132, 133, 134. and see if similar peaks are present
* Run UV-Vis on AuNP/BSA samples 120, 128, 130, 132, 133, 134. and see if similar peaks are present
* Run third UV-Vis on Tris buffer solutions of pH 8 and compare absorbances to the data collected last week.  
* Run third UV-Vis on Tris buffer solutions of pH 8 and compare absorbances to the data collected last week.  
==Procedure==
* UV-Vis was run for the AuNP/BSA samples as well as water to account for background noise.
* The third UV-Vis was run on the Tris buffer solutions at pH 8
* More AuNP/ BSA solution was made at a concentration of 170 and place in the oven for 4 hours at 80°C. These samples are to be used tomorrow and will be combined with the pH 10 tris buffer solution and have a UV-Vis run on them every hour.
==Data==
Link to spreadsheet and chart of redone UV-Vis:
*http://openwetware.org/wiki/Image:Chem-_Redone_AuNP-BSA.xlsx
==Conclusions==
For the redone AuNP/BSA UV-Vis tests done. Instead of just one peak at 550nm, there was an uncharacteristic peak at around 490-500nm as well. As a result, distilled water was also tested to see if it had been a factor in causing an adjustment to the same. In the data spreadsheet, the  UV-Vis data for each of the samples then had the UV-Vis data for water subtracted from it in order to test if it was the water that had caused the peak. The graphs clearly show that without the water, the background, the second peak was removed.
* Other observations were that compared to the original 14 solution data, the absorbency was much higher as compared to the retested 6 samples where the absorbency is smalled by an entire order of magnitude.


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Revision as of 10:47, 12 September 2012

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Day 4

Goals

  • Run UV-Vis on AuNP/BSA samples 120, 128, 130, 132, 133, 134. and see if similar peaks are present
  • Run third UV-Vis on Tris buffer solutions of pH 8 and compare absorbances to the data collected last week.

Procedure

  • UV-Vis was run for the AuNP/BSA samples as well as water to account for background noise.
  • The third UV-Vis was run on the Tris buffer solutions at pH 8
  • More AuNP/ BSA solution was made at a concentration of 170 and place in the oven for 4 hours at 80°C. These samples are to be used tomorrow and will be combined with the pH 10 tris buffer solution and have a UV-Vis run on them every hour.

Data

Link to spreadsheet and chart of redone UV-Vis:

Conclusions

For the redone AuNP/BSA UV-Vis tests done. Instead of just one peak at 550nm, there was an uncharacteristic peak at around 490-500nm as well. As a result, distilled water was also tested to see if it had been a factor in causing an adjustment to the same. In the data spreadsheet, the UV-Vis data for each of the samples then had the UV-Vis data for water subtracted from it in order to test if it was the water that had caused the peak. The graphs clearly show that without the water, the background, the second peak was removed.

  • Other observations were that compared to the original 14 solution data, the absorbency was much higher as compared to the retested 6 samples where the absorbency is smalled by an entire order of magnitude.





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