User:Rebeca Rodriguez/Notebook/Chem 581/2014/10/24: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Objective== | ==Objective== | ||
Make a column for column chromatography. | |||
==Description== | ==Description== | ||
# | # Column Packing | ||
## The column was made with a syringe with a stop cock at the bottom. | |||
## We packed the column with glass wool and washed it with methanol to help keep it packed. | |||
## A layer of sand made of rocks from outside (mortar and pestle) is added next | |||
## A solution of 9.9999g of Silica in methanol was poured into the column. | |||
##* One must wait until the column settles. | |||
## The column was washed with deionized water until the column ran clear. | |||
## 10mL of BPA and let it run into the layer of silica. | |||
## 10mL of water was run through the column, and then 30mL of methanol were run into the column | |||
##* These were divided into 8 5mL fractions. | |||
[[Category:Course]] | [[Category:Course]] |
Latest revision as of 00:32, 27 September 2017
Biomaterials Design Lab | Main project page Previous entry Next entry |
ObjectiveMake a column for column chromatography. Description
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