User:Samiye Yaman/Notebook/Lab 571/2011/09/28
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Entry titleObjectiveDescription- Two buffer solution (one with 50 mL of Tris and one with 50mL of Tris plus 1M of NaCl)was filtered. The one without NaCl was filtered first.
*Buffer A(50 mM of Tris) was loaded to the loop. *Protein was loaded to the loop. *Buffer B (50 mM of Tris+ 1M Nacl) was loaded to the loop. **Both Buffer A and B were covered with aluminum foil to prevent the entry of foreign particles. *Buffer B was run in order to get rid of the foreign particles inside the column. *Buffer A was run again to start the procedure. *10 ml The loop was injected to was the negatively charged particles in the column. *The gradient was run at 50% for 15min. *Fractions (5ml each) were collected. *The gradient was continued to run for 50% for 15 more mins again. *Fractions (5ml each)were collected. *The gradient was run at 50% for 25min. *Fractions (5ml each) were collected. *Rest of the protein was loaded to the loop. *The gradient was run with 100% in order to clean the column. *The fraction was run at 0. *Buffer B was run again. *After running Buffer B, Buffer A was run in order to reequilibrate.
Data
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