User:Samiye Yaman/Notebook/Lab 581/2012/10/17: Difference between revisions

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==Data==
==Data==
* Add data and results here...
 
'''ISE results for sodium sulfate dilutions'''
 
*Dilution 1(1.25ppm)= -180mV
 
*Dilution 2(2.5ppm)=  -182.6mV
 
*Dilution 3(5ppm)  = -182.4mV
 
*Dilution 4(10ppm )= -177.4mV
 
*Dilution 5(20ppm)= -174.6mV


==Notes==
==Notes==

Revision as of 20:14, 30 October 2012

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Objective

Description

Part 1 (ISE)

  • 0.1 ml of Sodium Ionic Strength Adjuster and 3 ml of each Sodium Sulfate solution was mixed in separate test tubes.
  • Channel 1 of the Orien Versa Star was used.
  • Before each measurement 30 sec was wait.


Part 2 Preparation of Porphyrin dilutions

  • Dilution 1: 1 ml of stock solution and 9 ml of HPLC water (concentration is 100 micM)
  • Dilution 2: 5 ml of dilution 1 and 5 ml of HPLC water (concentration is 50 micM)
  • Dilution 3: 5 ml of dilution 2 and 5 ml of HPLC water (concentration is 25 micM)
  • Dilution 4: 5 ml of dilution 3 and 5 ml of HPLC water (concentration is 12.5 micM)
  • Dilution 5: 5 ml of dilution 4 and 5 ml of HPLC water (concentration is 6.25 micM)

Data

ISE results for sodium sulfate dilutions

  • Dilution 1(1.25ppm)= -180mV
  • Dilution 2(2.5ppm)= -182.6mV
  • Dilution 3(5ppm) = -182.4mV
  • Dilution 4(10ppm )= -177.4mV
  • Dilution 5(20ppm)= -174.6mV

Notes

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