User:Samiye Yaman/Notebook/Lab 581/2012/10/17

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Objective

Description

Part 1 (ISE)

  • 0.1 ml of Sodium Ionic Strength Adjuster and 3 ml of each Sodium Sulfate solution was mixed in separate test tubes.
  • Channel 1 of the Orien Versa Star was used.
  • Before each measurement 30 sec was wait.


Part 2 Preparation of Porphyrin dilutions

  • Dilution 1: 1 ml of stock solution and 9 ml of HPLC water (concentration is 10 micM)
  • Dilution 2: 5 ml of dilution 1 and 5 ml of HPLC water (concentration is 5 micM)
  • Dilution 3: 5 ml of dilution 2 and 5 ml of HPLC water (concentration is 2.5 micM)
  • Dilution 4: 5 ml of dilution 3 and 5 ml of HPLC water (concentration is 1.25 micM)
  • Dilution 5: 5 ml of dilution 4 and 5 ml of HPLC water (concentration is 0.625 micM)

Data

ISE results for sodium sulfate dilutions

  • Dilution 1(1.25ppm)= -180mV
  • Dilution 2(2.5ppm)= -182.6mV
  • Dilution 3(5ppm) = -182.4mV
  • Dilution 4(10ppm )= -177.4mV
  • Dilution 5(20ppm)= -174.6mV


  • We decided detection limit was between dilution 1 and 2.
  • More negative number means less sodium ion in the solution.
  • The porphyrin films prepared on 10/10/2012 had a yellowish green color. They were not clear as the other films.

Notes

This area is for any observations or conclusions that you would like to note.


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