User:Samiye Yaman/Notebook/Lab 581/2013/03/27

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(Description)
(Description)
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==Description==
==Description==
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'''Part 1: Freeze-thaw vortex cycle of liposomes'''
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*5 freeze-thaw vortex cycle for liposome coated hydrogel. (vortex for 30sec, freeze (completely) by using liquid nitrogen then thaw (completely)in a water bath. Repeat the cycle 5 times.
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*Centrifuge the solution(4000rpm for 5 min) then remove the 25mM Tris solution from the epi-test tube.
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*Add 1.5 mL of 25mM Tris buffer back to the epi test tube with hydrogels.
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'''Part 2: Prepare a concentration range for R6G'''
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*Add 2mg of PVOH to 6 different epi test tubes.
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*Add 1.5 ml of phosphate buffer.
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*Add different volume of 1mM R6G to each epi test tube. (10μL, 15μL, 20μL, 25μL, 30μL, 35μL)
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==Data==
==Data==
==Notes==
==Notes==

Revision as of 10:18, 27 March 2013

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Contents

Objective

Description

Part 1: Freeze-thaw vortex cycle of liposomes

  • 5 freeze-thaw vortex cycle for liposome coated hydrogel. (vortex for 30sec, freeze (completely) by using liquid nitrogen then thaw (completely)in a water bath. Repeat the cycle 5 times.
  • Centrifuge the solution(4000rpm for 5 min) then remove the 25mM Tris solution from the epi-test tube.
  • Add 1.5 mL of 25mM Tris buffer back to the epi test tube with hydrogels.


Part 2: Prepare a concentration range for R6G

  • Add 2mg of PVOH to 6 different epi test tubes.
  • Add 1.5 ml of phosphate buffer.
  • Add different volume of 1mM R6G to each epi test tube. (10μL, 15μL, 20μL, 25μL, 30μL, 35μL)

Data

Notes

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