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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span>
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==Objectives==


==Description==
==Description==
*The course is designed by American University to experiment different aspects of PVOH films.


*During the course PVOH films with different molecular weight will be produced. Effects of cross-linking, addition of different molecules such as gluteraldehyde, different acids, porphrin will be also tested.
'''Part A: Preparing Dilutions for UV-Vis Measurements'''
 
*Centrifuge 6 solutions containing varying amounts of R6G prepared on 03/27/2013 for 5 minutes at 11200rpm 3 times.
 
*Prepare stock solutions for comprison (10μL, 15μL, 20μL, 25μL, 30μL and 35μL in 1.5 ml phosphate buffer)
 
* UV-Vis measurements of stock solutions and supernatants were taken
 
'''Part B: Observing pH changes'''
 
*Separate crystal violet containing hydrogels into two epi-test tubes, labelled as A & B.(app 1mg in each)
 
*Add 1mL of 1M HCl to one epi test tube A and 1mL NaOH to epitest tube B.
 
3) Centrifuge for 2 min at 11200rpm.
 
4) Take UV-Vis of supernatants
 
'''Part C: Prepare hydrogels with further pH analysis'''
 
*0.5mg samples of PVOH hydrogels containing no lecithin and no dye was added in to 3 seperate epi test tubes with 1.5 mL phosphate buffer.
 
==Data==
 
[[Image:Graf_10ul.png]]
 
[[Image:15ul.png]]
 
[[Image:20ul.png]]
 
[[Image:Graf_25ul.png]]
 
[[Image:30ul.png]]
 
[[Image:35ul.png]]


*UV-Vis spectrum, Atomic Absorption spectrum, X-Ray, FTIR, DMA, ISE and many other instruments will be used to analyze the results.
==Notes==

Latest revision as of 22:35, 26 September 2017

Experimental Chemistry Main project page
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Objectives

Description

Part A: Preparing Dilutions for UV-Vis Measurements

  • Centrifuge 6 solutions containing varying amounts of R6G prepared on 03/27/2013 for 5 minutes at 11200rpm 3 times.
  • Prepare stock solutions for comprison (10μL, 15μL, 20μL, 25μL, 30μL and 35μL in 1.5 ml phosphate buffer)
  • UV-Vis measurements of stock solutions and supernatants were taken

Part B: Observing pH changes

  • Separate crystal violet containing hydrogels into two epi-test tubes, labelled as A & B.(app 1mg in each)
  • Add 1mL of 1M HCl to one epi test tube A and 1mL NaOH to epitest tube B.

3) Centrifuge for 2 min at 11200rpm.

4) Take UV-Vis of supernatants

Part C: Prepare hydrogels with further pH analysis

  • 0.5mg samples of PVOH hydrogels containing no lecithin and no dye was added in to 3 seperate epi test tubes with 1.5 mL phosphate buffer.

Data

Notes
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