User:Spenser Skates/Notebook/pTKX luciferase biobrick/2008/04/28: Difference between revisions
From OpenWetWare
(Autocreate 2008/04/28 Entry for User:Spenser_Skates/Notebook/pTKX_luciferase_biobrick) |
|||
| Line 5: | Line 5: | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
== | ==Incorporation of sequence into BB backbone== | ||
Cut PCR results and Kan backbone seperately with EcoRI and PstI, then denatured restriction enzymes. Ligated DNA together with T4 DNA ligase.<br> | |||
[[Knight:DNA_ligation_using_T4_DNA_ligase]]<br> | |||
Transformed ligated product into E.coli by heatshock<br> | |||
[[Transforming_chemically_competent_cells_%28Inoue%29]]<br> | |||
<!-- ## Do not edit below this line unless you know what you are doing. ## --> | <!-- ## Do not edit below this line unless you know what you are doing. ## --> | ||
|} | |} | ||
Revision as of 06:45, 6 May 2008
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Incorporation of sequence into BB backboneCut PCR results and Kan backbone seperately with EcoRI and PstI, then denatured restriction enzymes. Ligated DNA together with T4 DNA ligase.
| |
