User:Spenser Skates/Notebook/pTKX luciferase biobrick/2008/04/30: Difference between revisions
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(Autocreate 2008/04/30 Entry for User:Spenser_Skates/Notebook/pTKX_luciferase_biobrick) |
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== | ==Miniprep, ran plasmid on gel== | ||
Performed a miniprep of the DNA extracted from E. coli, expecting to obtain as plasmid with insert+backbone<br> | |||
[[Miniprep/Qiagen_kit]]<br> | |||
Performed a restriction digest on the plasmid with EcoRI and PstI then denatured the restriction enzymes.<br> | |||
Ran on gel, found backbone without an insert (single piece of DNA 2.6kb in length).<br> | |||
Verified original plasmid had mutation added (removal of XbaI site in ORF). | |||
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Revision as of 07:02, 6 May 2008
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Miniprep, ran plasmid on gelPerformed a miniprep of the DNA extracted from E. coli, expecting to obtain as plasmid with insert+backbone
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