User:Stuart McKellar/Notebook/Bird Sex Testing/2013/01/15

From OpenWetWare
Jump to navigationJump to search
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Results

  • last samples produced valid results when compared with positive controls

Repeating experiment with 2550/2870 primer pair. Compare the photos and decide 100% which set I will be using.

Method: run a PCR @ 60°C and with 1ul 2550/2870 primers. Visualise on a 1% gel. 10 samples.

Master Mix: 7 x lorikeets 2 x positive controls (male female) 1 negative control

  • 1ul F primer
  • 1ul R primer
  • 2.0ul dNTPs
  • 0.5u Taq
  • 1ul DNA
  • 2.5ul 10x buffer
  • 1.5ul MgCl2
  • 16ul H2O
  • 1:1SWWBD
  • 2:2SWWBD
  • 3:R5#9
  • 4:R5#51
  • 5:R5#52
  • 6:R5#100
  • 7 :Pink Gold

male female neg



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Stuart_McKellar/Notebook/Bird_Sex_Testing/2013/01/15&oldid=667663"