User:Stuart McKellar/Notebook/Bird Sex Testing/2013/01/15
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Repeating experiment with 2550/2870 primer pair. Compare the photos and decide 100% which set I will be using.
Method: run a PCR @ 60°C and with 1ul 2550/2870 primers. Visualise on a 1% gel. 10 samples.
Master Mix: 7 x lorikeets 2 x positive controls (male female) 1 negative control
male female neg
Results: no bands. Try lower annealing temperature - say 55°C