User:Tara K. Luckau/Notebook/Team ConGen/2011/03/11: Difference between revisions

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:* use labelled forward primer (Scun2 F FAM) (get ready to genotype)
:* use labelled forward primer (Scun2 F FAM) (get ready to genotype)
:* full temperature and MgCl<sub>2</sub> gradient to find annealing temperature (no touchdown)
:* full temperature and MgCl<sub>2</sub> gradient to find annealing temperature (no touchdown)
* [[Image:2011 03 11 PCR.jpg|800 px]]
* [[Image:2011 03 11 PCR.jpg|900 px]]





Revision as of 15:40, 11 March 2011

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Scun2 FAM Gradient PCR

  • Moving on with Scun2:
  • use labelled forward primer (Scun2 F FAM) (get ready to genotype)
  • full temperature and MgCl2 gradient to find annealing temperature (no touchdown)


Subsection

  • here