User:Tara K. Luckau/Notebook/Team ConGen/2011/06/01: Difference between revisions
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===Gel=== | ===Gel=== | ||
====Pour==== | ====Pour==== | ||
* 270 mL 1x TAE | * 270 mL 1x TAE + 5.4 g agarose | ||
* microwave: set for 5 min @ half power, will first boil at 1:20, done about 0:20 | * microwave: set for 5 min @ half power, will first boil at 1:20, done about 0:20 | ||
* 27 µL GelRed | * 27 µL GelRed | ||
====Load==== | ====Load==== | ||
* 1 µL gel load dye | * 1 µL gel load dye + 5 µL PCR product | ||
* 6 µL ladder | * 6 µL ladder | ||
====Run==== | ====Run==== | ||
Revision as of 17:06, 1 June 2011
 Tara's Lab Notebook
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