User:Tara K. Luckau/Notebook/Team ConGen/2011/06/23: Difference between revisions
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! Pour !! Load !! Run | ! Pour !! Load !! Run | ||
|- | |- | ||
| 270 mL 1x TAE + 5.4 g agarose || | | 270 mL 1x TAE + 5.4 g agarose || 1 µL gel load dye + 5 µL PCR product || 160 V | ||
|- | |- | ||
| 27 µL GelRed || 6 µL ladder || | | 27 µL GelRed || 6 µL ladder || 50 minutes | ||
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==SCOC Multiplex 1== | ==SCOC Multiplex 1== | ||
* SCOC Multiplex 1 diversity panel with labelled primers to send to frag, part deux! | * SCOC Multiplex 1 diversity panel with labelled primers to send to frag, part deux! | ||
===PCR=== | ===PCR=== | ||
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* some of the samples used for the diversity panel are getting low ... should switch out soon | * some of the samples used for the diversity panel are getting low ... should switch out soon | ||
* SCOC TP3 X01 was low, like the others, but I already had a SCOC TP3 extracted last year in the Mass Extract ... so used it, SCOC TP3 X06 | * SCOC TP3 X01 was low, like the others, but I already had a SCOC TP3 extracted last year in the Mass Extract ... so used it, SCOC TP3 X06 | ||
===Gel=== | ===Gel=== | ||
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| 6 µL ladder || 1 hour | | 6 µL ladder || 1 hour | ||
|} | |} | ||
* see 13 June 2011 for banding pattern expectations | * see 13 June 2011 for banding pattern expectations | ||
* [[Image:20110623 Gel2| | * [[Image:20110623 Gel2.jpg|600 px]] | ||
* still a whole lotta nada :( | |||
* strong primer band, again | |||
* a bit of amplification of Scun9/10/11, but it's crap | |||
===Next Steps=== | |||
* try full gradient on multiplex, ugh | |||
Revision as of 09:28, 24 June 2011
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SCOC Multiplex 1PCR
Gel
SCOC Multiplex 1
PCR
Gel
Next Steps
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