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SCOC Multiplex 1
PCR
- similar to 13 June 2011 PCR - uniplexes of each primer pair, then multiplex, using labeled primers
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Gel
| Pour | Load | Run
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| 270 mL 1x TAE + 5.4 g agarose | 1 µL gel load dye + 5 µL PCR product | 160 V
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| 27 µL GelRed | 6 µL ladder | 50 minutes
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- see 13 June 2011 for banding pattern expectations
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- consistent with 13 June 2011 results (missing Scun22 band, but all others seem to be there)
- notice bright primer band on multiplex only - probably could do some optimization to make reaction more efficient
SCOC Multiplex 1
- SCOC Multiplex 1 diversity panel with labelled primers to send to frag, part deux!
PCR
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- some of the samples used for the diversity panel are getting low ... should switch out soon
- SCOC TP3 X01 was low, like the others, but I already had a SCOC TP3 extracted last year in the Mass Extract ... so used it, SCOC TP3 X06
Gel
- used left-over gel from 13 June 2011
| Load | Run
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| 2 µL gel load dye + 4 µL PCR product | 160 V
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| 6 µL ladder | 1 hour
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- see 13 June 2011 for banding pattern expectations
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- still a whole lotta nada :(
- strong primer band, again
- a bit of amplification of Scun9/10/11, but it's crap
Next Steps
- try full gradient on multiplex, ugh
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Tara's Lab Notebook
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