User:Tara K. Luckau/Notebook/Team ConGen/2011/06/23: Difference between revisions
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* consistent with 13 June 2011 results (missing Scun22 band, but all others seem to be there) | * consistent with 13 June 2011 results (missing Scun22 band, but all others seem to be there) | ||
* notice bright primer band on multiplex only - probably could do some optimization to make reaction more efficient | * notice bright primer band on multiplex only - probably could do some optimization to make reaction more efficient | ||
==SCOC Multiplex 1== | ==SCOC Multiplex 1== | ||
* SCOC Multiplex 1 diversity panel with labelled primers to send to frag, part deux! | * SCOC Multiplex 1 diversity panel with labelled primers to send to frag, part deux! | ||
===PCR=== | ===PCR=== | ||
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* some of the samples used for the diversity panel are getting low ... should switch out soon | * some of the samples used for the diversity panel are getting low ... should switch out soon | ||
* SCOC TP3 X01 was low, like the others, but I already had a SCOC TP3 extracted last year in the Mass Extract ... so used it, SCOC TP3 X06 | * SCOC TP3 X01 was low, like the others, but I already had a SCOC TP3 extracted last year in the Mass Extract ... so used it, SCOC TP3 X06 | ||
===Gel=== | ===Gel=== | ||
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| 6 µL ladder || 1 hour | | 6 µL ladder || 1 hour | ||
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* see 13 June 2011 for banding pattern expectations | * see 13 June 2011 for banding pattern expectations | ||
* [[Image:20110623 Gel2.jpg|600 px]] | * [[Image:20110623 Gel2.jpg|600 px]] | ||
* still a whole lotta nada :( | |||
* strong primer band, again | |||
* a bit of amplification of Scun9/10/11, but it's crap | |||
===Next Steps=== | |||
* try full gradient on multiplex, ugh | |||
Revision as of 09:28, 24 June 2011
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SCOC Multiplex 1PCR
Gel
SCOC Multiplex 1
PCR
Gel
Next Steps
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