User:Tara K. Luckau/Notebook/Team ConGen/2011/06/24

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SCOC Multiplex 1

PCR

  • multiplex looks good in some PCRs (when run next to uniplexes), but not in others (when scaled up for frag submission)
  • try full gradient (temperature and buffer) of multiplexed reaction


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 1 µL gel load dye + 5 µL PCR product 160 V
27 µL GelRed 6 µL ladder 50 minutes




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