User:Tara K. Luckau/Notebook/Team ConGen/2011/10/24: Difference between revisions
From OpenWetWare
No edit summary |
(→PCR) |
||
(4 intermediate revisions by the same user not shown) | |||
Line 9: | Line 9: | ||
==CNHY | ==CNHY Acos6== | ||
Line 19: | Line 19: | ||
! Pour !! Load !! Run | ! Pour !! Load !! Run | ||
|- | |- | ||
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 80 V | | 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V ... 80 V | ||
|- | |- | ||
| 27 µL GelRed || 6 µL ladder || | | 27 µL GelRed || 6 µL ladder || 25 min ... 1.5 hr | ||
|} | |} | ||
Line 27: | Line 27: | ||
[[Image:20111024_Gel.png|800 px]] | [[Image:20111024_Gel.png|800 px]] | ||
* | * lots of mispriming (fuzzy bands because had to let run for nearly 2 hours), but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions | ||
==CNHY Ai5013== | ==CNHY Ai5013== | ||
Line 34: | Line 33: | ||
===PCR=== | ===PCR=== | ||
* [[Image: | * [[Image:20111024_PCR.png|800 px]] | ||
Revision as of 13:06, 27 October 2011
Tara's Lab Notebook | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||
CNHY Acos6Gel
CNHY Ai5013PCR
|