User:Tara K. Luckau/Notebook/Team ConGen/2012/01/14: Difference between revisions
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===PCR=== | ===PCR - Uniplexes=== | ||
* uniplex to determine marker range | |||
* individual CNHY and CNTI DNAs to determine marker quality when uniplexed | |||
* pooled CNHY and CNTI DNAs to determine marker range when uniplexed | |||
* [[Image:20120114_PCRa.png|800 px]] | * [[Image:20120114_PCRa.png|800 px]] | ||
===PCR=== | ===PCR - Multiplex all-in-one=== | ||
* multiplex to determine general marker quality and strength | |||
* individual CNHY and CNTI DNAs to determine marker quality when multiplexed | |||
* pooled CNHY and CNTI DNAs to determine marker range when multiplexed | |||
* [[Image:20120114_PCRb.png|800 px]] | * [[Image:20120114_PCRb.png|800 px]] | ||
===PCR=== | ===PCR - Multiplex A and Multiplex B=== | ||
* split multiplex into two (no post-PCR mixing) based on primer interactions, gradient results, and estimated amplicon size | |||
* compare all-in-one multiplex with Multiplex A and B - better? | |||
* compare scores in multiplex A and B with uniplex to make sure scores match and nothing weird is happening | |||
* [[Image:20120114_PCRc.png|800 px]] | * [[Image:20120114_PCRc.png|800 px]] | ||
Revision as of 20:29, 14 January 2012
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CNHY / CNTI MultiplexPCR - Uniplexes
PCR - Multiplex all-in-one
PCR - Multiplex A and Multiplex B
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