User:Tara K. Luckau/Notebook/Team ConGen/2012/03/28: Difference between revisions

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==CNTI Acos5==
==SCOC and CNHY Multiplex Optimization==


gel from 4 PCRs done on 27 March 2012


===PCR===
 
* [[Image:20120110_PCRa.png|800 px]]
===Gel===
 
used pre-poured gel sitting in fridge (unknown pour date)




===Gel===
[[Image:20120328_Gel.png|500 px]]
 
* good - send to frag!
 
 
==Fragment Analysis Submission==


{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}


* [[Image:20120328 Frag.png|1000 px]]


[[Image:20120110_Gela.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
* FedEx Tracking# [http://www.fedex.com/Tracking?language=english&cntry_code=us&tracknumbers=876927708800 876927708800]
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
:: [[Image:20120328_FedEx.png|600 px]]
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
* UAGC Submission# JBME




* FedEx picked up 5:09pm, 28 March
* FedEx delivered 8:55am, 29 March
* UAGC received 1:18pm, 29 March
* UAGC completed 10:09am, 30 March




Revision as of 13:36, 30 March 2012

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SCOC and CNHY Multiplex Optimization

gel from 4 PCRs done on 27 March 2012


Gel

used pre-poured gel sitting in fridge (unknown pour date)


  • good - send to frag!


Fragment Analysis Submission


  • UAGC Submission# JBME


  • FedEx picked up 5:09pm, 28 March
  • FedEx delivered 8:55am, 29 March
  • UAGC received 1:18pm, 29 March
  • UAGC completed 10:09am, 30 March



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