User:Tara K. Luckau/Notebook/Team ConGen/2012/07/18: Difference between revisions

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==CNTI Acos5==
==SCOC MP1, CNHY/TI MP1==




===PCR===
===Gel===
* [[Image:20120110_PCRa.png|800 px]]


(used previously-made gel)


===Gel===
{| {{table}} style="text-align: center"
|-
! Pour !! Load !! Run
|-
| 270 mL 1x TAE + 5.4 g agarose || 2 µL gel load dye + 4 µL PCR product || 160 V
|-
| 27 µL GelRed || 6 µL ladder || 45 minutes
|}


[[Image:20120718_Gel.png|900 px]]


[[Image:20120110_Gela.png|800 px]]


* mispriming everywhere - primer pair not usable for CNHY
* SCOC MP1-G+Y
* lots of mispriming, but may be able to use conditions indicated by orange face ([[Image:orangefrownyface.png|20 px]]), if needed, under stringent conditions
:: bands present - send to frag
* favorable conditions indicated by [[Image:greenhappyface.png|25 px]]
* CNHY/TI MP1 Uniplex Ai5033
:: clear, clean band present - send to frag
* CNHY/TI MP1a
:: multiple bands present, though bright primer banding also occurs - send to frag
*CNHY/TI MP1b
:: multiple bands present, though bright primer banding also occurs - send to frag




Revision as of 15:07, 19 July 2012

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SCOC MP1, CNHY/TI MP1

Gel

(used previously-made gel)



  • SCOC MP1-G+Y
bands present - send to frag
  • CNHY/TI MP1 Uniplex Ai5033
clear, clean band present - send to frag
  • CNHY/TI MP1a
multiple bands present, though bright primer banding also occurs - send to frag
  • CNHY/TI MP1b
multiple bands present, though bright primer banding also occurs - send to frag




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