User:Tara K. Luckau/Notebook/Team ConGen/Entry Base: Difference between revisions
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==25 October 2010 - Ladder Mix== | ==25 October 2010 - Ladder Mix== | ||
===Manufacturer specifications=== | ===Manufacturer specifications=== | ||
* 0.1µg ladder / mm width of well | * 0.1µg ladder / mm width of well | ||
* STK at 1.0µg/µL | |||
===Other Knowns=== | |||
* well width (24-tooth comb) = 6mm | |||
* Jeremy (Bohonak lab) uses 5µL PCR product + 1.5µL dye | |||
===Calculations=== | ===Calculations=== | ||
* 6mm x (0.1µg ladder)/(1mm width) x (1µL/1µg) = 0.6µL ladder per well | |||
* 6mm x | * Per well: 0.6µL ladder + 4.4µL H<sub>2</sub>O + 1.5µL loading dye = 6.5µL total ladder mix | ||
* (loading dye = xylene cyanol and bromophenol, made by Bohonak lab) | |||
* Per well: 0.6µL ladder + 4.4µL H<sub>2</sub>O = 5µL ladder mix | ===Make Ladder Mix=== | ||
* (xylene and bromophenol | * 120µL ladder + 880µL H<sub>2</sub>O + 300µL loading dye = 1300µL ladder mix | ||
Revision as of 16:23, 25 October 2010
Tara's Lab Notebook | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page |
27 September 2010 - Ordered Primers
11 October 2010 - PCR Thermalcycler TestingPurpose
Results
Purpose
Results
12 October 2010 - PCR Thermalcycler TestingPurpose
Results
18 October 2010 - PCR Thermalcycler TestingPurpose
Results
18 October 2010 - Primer Rehydration, Scun2Purpose
Calculations
18 October 2010 - dNTP MixPurpose
Calculations
Wet Work
19 October 2010 - PCR Scun2 F-R21 October 2010 - Gel Scun2 F-RMake 1x TAE
Cast Gel
Next Try
25 October 2010 - Gel Scun2 F-RCast Gel
25 October 2010 - Ladder MixManufacturer specifications
Other Knowns
Calculations
Make Ladder Mix
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