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CNTI Acos5
PCR
- Image:20130110 PCRa.png
Gel
| Pour | Load | Run
|
| 270 mL 1x TAE + 5.4 g agarose | 2 µL gel load dye + 4 µL PCR product | 160 V
|
| 27 µL GelRed | 6 µL ladder | 45 minutes
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- Image:20130110 Gela.png
- mispriming everywhere - primer pair not usable for CNHY
- lots of mispriming, but may be able to use conditions indicated by orange face (
), if needed, under stringent conditions
- favorable conditions indicated by
Fragment Analysis Submission
- Image:20130227 Frag.png
- Image:20130227 FedEx.png
- FedEx picked up ____pm, 26 February
- FedEx delivered ____am, 27 February
- UAGC received ____am, 27 February
- UAGC completed ____am, 28 February
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