User:Tara K. Luckau/Notebook/Team ConGen/Entry Base: Difference between revisions
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|style="background-color: # | |style="background-color: #BCED91"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Tara's Lab Notebook</span> | ||
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== | ==27 September 2010 - Ordered Primers== | ||
* | * Ordered first set of primers | ||
* Scun2 and Scun22 (highly variable simple motifs) | |||
** Lance et al (2009) Conservation Genetics Resources, Sceloporus undulatus | |||
* Forward and reverse as given in publication, also reverse with pigtail (GTTTCTT) at 5' end, also forward with M13F(-21) tag (TGTAAAACGACGGCCAGT) | |||
* [[Image:Luckau_Primer20100927.jpg|500 px]] | |||
* Try all four combinations | |||
# F - R | |||
# F - R pigtail | |||
# F M13 - R | |||
# F M13 - R pigtail | |||
* If they don't hinder efforts of change optimization decisions, then may make optimization of multiplexes cheaper! | |||
==11 October 2010 - PCR Thermalcycler Testing== | |||
Revision as of 12:09, 25 October 2010
 Tara's Lab Notebook
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page |
27 September 2010 - Ordered Primers
11 October 2010 - PCR Thermalcycler Testing | |
