User:Tara K. Luckau/Notebook/Team ConGen/Entry Base: Difference between revisions
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* H6, H7 evaporated, but I think it's workable | * H6, H7 evaporated, but I think it's workable | ||
* Plan to do first optimization PCR tomorrow! | * Plan to do first optimization PCR tomorrow! | ||
==18 October 2010 - Primer Rehydration, Scun2 | ==18 October 2010 - Primer Rehydration, Scun2== | ||
===Purpose=== | ===Purpose=== | ||
* Rehydrate Scun2 primers for testing tomorrow | * Rehydrate Scun2 primers for testing tomorrow | ||
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* Aliquots: 1000µL into each of 5 snap-caps; 4 stored in freezer, 1 stored in fridge for immediate use | * Aliquots: 1000µL into each of 5 snap-caps; 4 stored in freezer, 1 stored in fridge for immediate use | ||
==19 October 2010 - PCR Scun2 F-R== | ==19 October 2010 - PCR Scun2 F-R== | ||
[[Image:Luckau_PCR20101019.jpg]] | * [[Image:Luckau_PCR20101019.jpg|500 px]] | ||
* DNA = SCOC CPN 82 | * DNA = SCOC CPN 82 | ||
* 2 mats with lab tape, no evaporation! | * 2 mats with lab tape, no evaporation! |
Revision as of 12:35, 25 October 2010
Tara's Lab Notebook | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page |
27 September 2010 - Ordered Primers
11 October 2010 - PCR Thermalcycler TestingPurpose
Results
Purpose
Results
12 October 2010 - PCR Thermalcycler TestingPurpose
Results
18 October 2010 - PCR Thermalcycler TestingPurpose
Results
18 October 2010 - Primer Rehydration, Scun2Purpose
Calculations
18 October 2010 - dNTP MixPurpose
Calculations
Wet Work
19 October 2010 - PCR Scun2 F-R21 October 2010 - Gel Scun2 F-RMake 1x TAE
Cast Gel
Next Try
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