User:Tk/Notebook/MF/2008/09/14: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(Autocreate 2008/09/14 Entry for User:Tk/Notebook/MF) |
(→Title) |
||
Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==In-Fusion protein identification== | ||
* | * Re-run In-Fusion gel | ||
** 10% Bis-tris gel | |||
** 150 V, 1 hr 20 min | |||
** prerun 30 min | |||
** dissolve In-Fusion mix in 13 ul water | |||
** Add to serial dilution mix of: | |||
*** 2 ul beta-mercaptoethanol | |||
*** 5 ul LDS sample buffer | |||
** 3 additional mixes of BME + LDS + 13 ul water | |||
** 2x serial dilutions into the LDS mix | |||
** incubate 15 min at 70C | |||
** run 10 ul SeeBlue plus 2 marker lanes | |||
** run 20 ul of each dilution | |||
** wash 2x with microwaved DI water 10 min | |||
** Add Simply Blue stain covering gel, incubate overnight | |||
** Wash 2x with hot DI water + kimwipes | |||
** Image with gel imager in water | |||
** store in plastic bag | |||
Revision as of 15:29, 14 September 2008
Mesoplasma florum simplification | <html><img src="/images/9/94/Report.png" border="0" /></html> Main Page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
In-Fusion protein identification
|