User:Trisha I. Ibeh/Notebook/Trisha Notebook/2014/01/29: Difference between revisions
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|style="background-color: #EEE"|[[Image: | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Objective== | ||
* | |||
==Procedure== | |||
==Magnetic Nanoparticles Day 6== | |||
*Mag BSA-AuNPs were created and bubbled through with nitrogen | |||
*UV Vis was performed on the samples made in the previous lab | |||
Create magnetic BSA-iron NPS | |||
# Synthesize BSA- iron NPs into test tube that will contain a total of 5mL | |||
# BSA- iron solution that has final ratio of 130:1 | |||
# Add 30 mM FeSO4*7H2O, 400 mM KOH, and 100 mM KNO3 | |||
# Water up to 5mL | |||
# De-gas 130 ratio of hemoglobin- iron solution with nitrogen for 4 hrs at 90 degrees Celsius | |||
Separately make Hemoglobin- AuNPs the same way as in steps 1-3 but instead of using nitrogen, put test tubes in incubator for 4 hrs at 80 degrees Celsius with ratios of: | |||
#125:1 | |||
#130:1 | |||
#135:1 | |||
==Notes== | |||
Hemoglobin could not dissolve in water therefore PIs continued with BSA | |||
Latest revision as of 23:41, 26 September 2017
Biomaterials Design Lab | Main project page Previous entry Next entry |
ObjectiveProcedureMagnetic Nanoparticles Day 6
Create magnetic BSA-iron NPS
NotesHemoglobin could not dissolve in water therefore PIs continued with BSA
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