Objective
Procedure
Magnetic Nanoparticles Day 6
- Mag BSA-AuNPs were created and bubbled through with nitrogen
- UV Vis was performed on the samples made in the previous lab
Create magnetic BSA-iron NPS
- Synthesize BSA- iron NPs into test tube that will contain a total of 5mL
- BSA- iron solution that has final ratio of 130:1
- Add 30 mM FeSO4*7H2O, 400 mM KOH, and 100 mM KNO3
- Water up to 5mL
- De-gas 130 ratio of hemoglobin- iron solution with nitrogen for 4 hrs at 90 degrees Celsius
Separately make Hemoglobin- AuNPs the same way as in steps 1-3 but instead of using nitrogen, put test tubes in incubator for 4 hrs at 80 degrees Celsius with ratios of:
- 125:1
- 130:1
- 135:1
Notes
Hemoglobin could not dissolve in water therefore PIs continued with BSA
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Biomaterials Design Lab
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