User:Trisha I. Ibeh/Notebook/Trisha Notebook/2014/01/29: Difference between revisions

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Objective==
==Procedure==
==Magnetic Nanoparticles Day 6==
==Magnetic Nanoparticles Day 6==
*Mag BSA-AuNPs were created and bubbled through with nitrogen
*Mag BSA-AuNPs were created and bubbled through with nitrogen


*UV Vis was performed on the samples made in the previous lab
*UV Vis was performed on the samples made in the previous lab
Create magnetic BSA-iron NPS
# Synthesize BSA- iron NPs into test tube that will contain a total of 5mL
#      BSA- iron solution that has final ratio of 130:1
# Add 30 mM FeSO4*7H2O, 400 mM KOH, and 100 mM KNO3
# Water up to 5mL
# De-gas 130 ratio of hemoglobin- iron solution with nitrogen for 4 hrs at 90 degrees Celsius
Separately make Hemoglobin- AuNPs the same way as in steps 1-3 but instead of using nitrogen, put test tubes in incubator for 4 hrs at 80 degrees Celsius with ratios of:
#125:1
#130:1
#135:1


==Notes==
==Notes==

Latest revision as of 23:41, 26 September 2017

Biomaterials Design Lab Main project page
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Objective

Procedure

Magnetic Nanoparticles Day 6

  • Mag BSA-AuNPs were created and bubbled through with nitrogen
  • UV Vis was performed on the samples made in the previous lab

Create magnetic BSA-iron NPS

  1. Synthesize BSA- iron NPs into test tube that will contain a total of 5mL
  2. BSA- iron solution that has final ratio of 130:1
  3. Add 30 mM FeSO4*7H2O, 400 mM KOH, and 100 mM KNO3
  4. Water up to 5mL
  5. De-gas 130 ratio of hemoglobin- iron solution with nitrogen for 4 hrs at 90 degrees Celsius



Separately make Hemoglobin- AuNPs the same way as in steps 1-3 but instead of using nitrogen, put test tubes in incubator for 4 hrs at 80 degrees Celsius with ratios of:

  1. 125:1
  2. 130:1
  3. 135:1

Notes

Hemoglobin could not dissolve in water therefore PIs continued with BSA




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