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3/8/13
- Ligation was repeated.
- Attempted to grow more liquid cultures of ligated plasmids.
- Attempted to confirm ligation by streaking the colonies on an agar plate.
- All attempts proved unsuccessful.
| Reagent |
Volume |
|
15 μL/lane, 1% agarose; Ladder
|
| DNA (plasmid) |
20 μL
|
| 10x buffer |
3.0
|
| Enzyme 1 (Pst1) |
1.0
|
| Enzyme 2 (Spe1 or Xba1) |
1.0
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| dH2O |
5.0 μL
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| |
30 μL --> 37°C/ ~15 min.
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