User:Wesley J. Houston/Notebook/MAP/2013/03/07

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry


  • Prepped plasmids using Zyppy miniprep kit.
  • Digested plasmids with EcoR1 and Pst1.
  • Ran gel electrophoresis to confirm assembly.
Reagent Volume   Results of the ligation electrophoresis
15 μL/lane, 1% agarose; Ladder
DNA (plasmid) 20 μL
10x buffer 3.0
EcoR1 1.0
Pst1 1.0
dH2O 5.0 μL
  30 μL --> 37°C/ ~15 min.

  • No DNA present, will repeat ligation.
    • Determined to be a false positive.

Personal tools