User:Wesley J. Houston/Notebook/MAP/2013/03/07

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3/7/13 Prepped plasmids using Zyppy miniprep kit. Digested plasmids with EcoR1 and Pst1. Ran gel electrophoresis to confirm assembly. Reagent Volume   15 μL/lane, 1% agarose; Ladder DNA (plasmid) 20 μL 10x buffer 3.0 EcoR1 1.0 Pst1 1.0 dH2O 5.0 μL   30 μL --> 37°C/ ~15 min. No DNA present, will repeat ligation. Determined to be a false positive.