User:Wesley J. Houston/Notebook/MAP/2013/03/26: Difference between revisions
From OpenWetWare
(Autocreate 2013/03/26 Entry for User:Wesley_J._Houston/Notebook/MAP) |
(fix raw html notebook nav) |
||
(3 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==3/26/13== | ||
* | * Prepped plasmids of KAH182 and KAH201 liquid cultures, following the Zyppy plasmid miniprep kit. | ||
* Determined yields of plasmids. | |||
{| class="wikitable" border="0" cellspacing="3" <!-- [DNA] table --> | |||
|- | |||
| <u>Sample</u> || <u>OD260</u> || <u>260/280</u> || <u>ng/μL</u> | |||
|- | |||
| 1. KAH201 (Plasmid) || 0.056 || 1.732 || 56.395 | |||
|- | |||
| 2. KAH201 (Plasmid) || 0.069 || 1.857 || 69.164 | |||
|} | |||
{| class="wikitable" border="0" cellspacing="3" <!-- Digest rxn. table --> | |||
|-valign="top" | |||
| <u>Reagent</u> || <u>Volume</u> || | |||
| rowspan="9" | [[Image:Electrophoresis6.jpg |150px|Results of the fifth electrophoresis attempt]]<br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | |||
|- | |||
| DNA (plasmid) || 25 μL | |||
|- | |||
| 10x buffer || 3.0 | |||
|- | |||
| enzyme 1 (Pst1) || 1.0 | |||
|- | |||
| enzyme 2 (Xba1 or Spe1)|| 1.0 | |||
|- | |||
| dH<sub>2</sub>O || 0.0 μL | |||
|- | |||
| || 30 μL --> 37°C/ ~15 min. | |||
|} | |||
> Measure conc.'s of gel purified fragments (KAH182 = lanes 2 and 3; KAH201 = lanes 4 and 5) | |||
{| class="wikitable" border="0" cellspacing="3" <!-- [DNA] table --> | |||
|- | |||
| <u>Sample</u> || <u>OD260</u> || <u>260/280</u> || <u>ng/μL</u> | |||
|- | |||
| 1. KAH201 (S/P) || 0.029 || 1.054 || 29.262 | |||
|- | |||
| 2. KAH201 (S/P) || 0.006 || 1.381 || 6.172 | |||
|- | |||
| 3. KAH182 (X/P) || -0.002 || 2 || -1.702 | |||
|- | |||
| 4. KAH182 (X/P) || 0.007 || 5.583 || 7.129 | |||
|} | |||
Latest revision as of 22:35, 26 September 2017
Project name | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||||||||||||||
3/26/13
|