User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/02/17: Difference between revisions
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* Supernatant is stored @ -20 °C and used for ELISA later on | * Supernatant is stored @ -20 °C and used for ELISA later on | ||
* Remaining cells are used for viability staining. (See [[ Alamar Blue staining]]) | * Remaining cells are used for viability staining. (See [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/17#Alamar_Blue_staining| Alamar Blue staining]]) | ||
====Alamar Blue staining==== | ====Alamar Blue staining==== | ||
* Remove remaining medium from 24-wells plates | * Remove remaining medium from 24-wells plates | ||
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==Results== | ==Results== | ||
===Alamar Blue=== | |||
{| border="1" | |||
|+'''D9 (passage 21)''' | |||
!#1 | |||
!<u>1</u><BR>HT31P | |||
!<u>2</u><BR>HT31P | |||
!<u>3</u><BR>HT31P | |||
!<u>4</u><BR>HT31 | |||
!<u>5</u><BR>HT31 | |||
!<u>6</u><BR>HT31 | |||
|- | |||
!A (CTR) | |||
|style="background:red" |1824 | |||
|style="background:red" |1982 | |||
|style="background:red" |1953 | |||
|style="background:green" |5125 | |||
|style="background:green" |5172 | |||
|style="background:green" |5939 | |||
|- | |||
!B (CSE) | |||
|style="background:red" |956 | |||
|style="background:red" |964 | |||
|style="background:red" |785 | |||
|style="background:green" |4465 | |||
|style="background:green" |5006 | |||
|style="background:green" |5815 | |||
|- | |||
!C (cse +8p) | |||
|style="background:red" |1075 | |||
|style="background:red" |1104 | |||
|style="background:red" |971 | |||
|style="background:green" |5017 | |||
|style="background:green" |5241 | |||
|style="background:green" |5526 | |||
|- | |||
!D (cse+6Bnz) | |||
|style="background:red" |761 | |||
|style="background:red" |674 | |||
|style="background:red" |645 | |||
|style="background:green" |4408 | |||
|style="background:green" |5385 | |||
|style="background:green" |4943 | |||
|- | |||
|} | |||
<BR> | |||
{| border="1" | |||
|+'''D9 (passage 21)''' | |||
!#2 | |||
!<u>1</u><BR>HT31P | |||
!<u>2</u><BR>HT31P | |||
!<u>3</u><BR>HT31P | |||
!<u>4</u><BR>HT31 | |||
!<u>5</u><BR>HT31 | |||
!<u>6</u><BR>HT31 | |||
|- | |||
!A (CTR) | |||
|style="background:red" |2232 | |||
|style="background:red" |2560 | |||
|style="background:red" |2506 | |||
|style="background:green" |6431 | |||
|style="background:green" |6394 | |||
|style="background:green" |7264 | |||
|- | |||
!B (CSE) | |||
|style="background:red" |1113 | |||
|style="background:red" |1050 | |||
|style="background:red" |998 | |||
|style="background:green" |6077 | |||
|style="background:green" |6513 | |||
|style="background:green" |7171 | |||
|- | |||
!C (cse +8p) | |||
|style="background:red" |1224 | |||
|style="background:red" |1024 | |||
|style="background:red" |1234 | |||
|style="background:green" |5606 | |||
|style="background:green" |6203 | |||
|style="background:green" |7559 | |||
|- | |||
!D (cse+6Bnz) | |||
|style="background:red" |730 | |||
|style="background:red" |826 | |||
|style="background:red" |858 | |||
|style="background:green" |5309 | |||
|style="background:green" |4856 | |||
|style="background:green" |2518 | |||
|- | |||
|} | |||
<BR> | |||
{| border="1" | |||
|+'''D9 (passage 21)''' | |||
!#3 | |||
!<u>1</u><BR>HT31P | |||
!<u>2</u><BR>HT31P | |||
!<u>3</u><BR>HT31P | |||
!<u>4</u><BR>HT31 | |||
!<u>5</u><BR>HT31 | |||
!<u>6</u><BR>HT31 | |||
|- | |||
!A (CTR) | |||
|style="background:red" |2359 | |||
|style="background:red" |2494 | |||
|style="background:red" |2434 | |||
|style="background:green" |5865 | |||
|style="background:green" |6713 | |||
|style="background:green" |6843 | |||
|- | |||
!B (CSE) | |||
|style="background:red" |998 | |||
|style="background:red" |929 | |||
|style="background:red" |957 | |||
|style="background:green" |5129 | |||
|style="background:green" |5632 | |||
|style="background:green" |6518 | |||
|- | |||
!C (cse +8p) | |||
|style="background:red" |946 | |||
|style="background:red" |1033 | |||
|style="background:red" |1026 | |||
|style="background:green" |5562 | |||
|style="background:green" |5622 | |||
|style="background:green" |6125 | |||
|- | |||
!D (cse+6Bnz) | |||
|style="background:red" |940 | |||
|style="background:red" |1123 | |||
|style="background:red" |982 | |||
|style="background:green" |6428 | |||
|style="background:green" |5590 | |||
|style="background:green" |4116 | |||
|- | |||
|} | |||
<BR> | |||
{| border="1" | |||
|+'''D12 (passage 27)''' | |||
!#1 | |||
!<u>1</u><BR>HT31P | |||
!<u>2</u><BR>HT31P | |||
!<u>3</u><BR>HT31P | |||
!<u>4</u><BR>HT31 | |||
!<u>5</u><BR>HT31 | |||
!<u>6</u><BR>HT31 | |||
|- | |||
!A (CTR) | |||
|style="background:red" |2151 | |||
|style="background:red" |2049 | |||
|style="background:red" |2021 | |||
|style="background:green" |7501 | |||
|style="background:green" |7411 | |||
|style="background:green" |7507 | |||
|- | |||
!B (CSE) | |||
|style="background:red" |773 | |||
|style="background:red" |848 | |||
|style="background:red" |814 | |||
|style="background:green" |5447 | |||
|style="background:green" |6630 | |||
|style="background:green" |6700 | |||
|- | |||
!C (cse +8p) | |||
|style="background:red" |718 | |||
|style="background:red" |769 | |||
|style="background:red" |769 | |||
|style="background:green" |4324 | |||
|style="background:green" |4835 | |||
|style="background:green" |6349 | |||
|- | |||
!D (cse+6Bnz) | |||
|style="background:red" |877 | |||
|style="background:red" |874 | |||
|style="background:red" |1002 | |||
|style="background:green" |5387 | |||
|style="background:green" |7649 | |||
|style="background:green" |6039 | |||
|- | |||
|} | |||
<BR> | |||
{| border="1" | |||
|+'''D12 (passage 27)''' | |||
!#2 | |||
!<u>1</u><BR>HT31P | |||
!<u>2</u><BR>HT31P | |||
!<u>3</u><BR>HT31P | |||
!<u>4</u><BR>HT31 | |||
!<u>5</u><BR>HT31 | |||
!<u>6</u><BR>HT31 | |||
|- | |||
!A (CTR) | |||
|style="background:red" |1389 | |||
|style="background:red" |2124 | |||
|style="background:red" |1980 | |||
|style="background:green" |7432 | |||
|style="background:green" |7069 | |||
|style="background:green" |7977 | |||
|- | |||
!B (CSE) | |||
|style="background:red" |925 | |||
|style="background:red" |1021 | |||
|style="background:red" |972 | |||
|style="background:green" |4957 | |||
|style="background:green" |5760 | |||
|style="background:green" |6746 | |||
|- | |||
!C (cse +8p) | |||
|style="background:red" |960 | |||
|style="background:red" |1030 | |||
|style="background:red" |1069 | |||
|style="background:green" |5381 | |||
|style="background:green" |4675 | |||
|style="background:green" |6522 | |||
|- | |||
!D (cse+6Bnz) | |||
|style="background:red" |767 | |||
|style="background:red" |877 | |||
|style="background:red" |1138 | |||
|style="background:green" |5216 | |||
|style="background:green" |6143 | |||
|style="background:green" |6754 | |||
|- | |||
|} | |||
<BR> | |||
{| border="1" | |||
|+'''D12 (passage 27)''' | |||
!#3 | |||
!<u>1</u><BR>HT31P | |||
!<u>2</u><BR>HT31P | |||
!<u>3</u><BR>HT31P | |||
!<u>4</u><BR>HT31 | |||
!<u>5</u><BR>HT31 | |||
!<u>6</u><BR>HT31 | |||
|- | |||
!A (CTR) | |||
|style="background:red" |2071 | |||
|style="background:red" |2158 | |||
|style="background:red" |2331 | |||
|style="background:green" |7252 | |||
|style="background:green" |7248 | |||
|style="background:green" |7881 | |||
|- | |||
!B (CSE) | |||
|style="background:red" |1098 | |||
|style="background:red" |953 | |||
|style="background:red" |1087 | |||
|style="background:green" |5078 | |||
|style="background:green" |5458 | |||
|style="background:green" |5958 | |||
|- | |||
!C (cse +8p) | |||
|style="background:red" |926 | |||
|style="background:red" |1324 | |||
|style="background:red" |1106 | |||
|style="background:green" |4599 | |||
|style="background:green" |4899 | |||
|style="background:green" |5503 | |||
|- | |||
!D (cse+6Bnz) | |||
|style="background:red" |908 | |||
|style="background:red" |738 | |||
|style="background:red" |957 | |||
|style="background:green" |4963 | |||
|style="background:green" |4311 | |||
|style="background:green" |6101 | |||
|- | |||
|} | |||
<BR> | |||
==Conclusion== | ==Conclusion== | ||
* | Samples should have been been into the wells at random to prevent the cells to concentrate in the first samples. | ||
===Related entries=== | |||
====Run 1: Ht31/Ht31P D9/D12==== | |||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/10|Splitting cells 10Feb2010]] | |||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/12|Counting cells 12Feb2010]] | |||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/15|Putting to S<sup>0</sup> 15Feb2010]] | |||
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/16|Stimulating cells 16Feb2010]] | |||
==Related topics== | ==Related topics== |
Revision as of 14:17, 18 March 2010
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Viability staining | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Summary
Materials & MethodsMaterialsMethodPreparations ELISA
Alamar Blue staining
ResultsAlamar Blue
ConclusionSamples should have been been into the wells at random to prevent the cells to concentrate in the first samples. Related entriesRun 1: Ht31/Ht31P D9/D12
Related topics20.109(S08):Testing_cell_viability_(Day3)
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