User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/02/26: Difference between revisions

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{|{{table}} width="800"
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">ELISA & Counting splitting cells</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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===Method===
===Method===
====ELISA====
* Same actions as [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/18|18Feb2010]]
{| border="1"
{| border="1"
|+'''6-wells'''
|+'''Sample dilution ELISA (Dilution buffer (μL)/Sample (μL))'''
!#
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!<u>1</u>
!<u>2</u>
!<u>3</u>
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!A
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!B
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{| border="1"
|+'''24-wells'''
!#
!<u>1</u>
!<u>1</u>
!<u>2</u>
!<u>2</u>
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!<u>6</u>
!<u>6</u>
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!A
|A
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|style="background:red"|100/100
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|style="background:red"|100/100
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|style="background:red"|100/100
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|style="background:red"|100/100
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|style="background:red"|100/100
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|style="background:red"|100/100
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!B
|B
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|style="background:green"|150/50
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|style="background:green"|150/50
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|style="background:green"|150/50
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|style="background:blue; color:white" |175/25
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|style="background:blue; color:white" |175/25
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|style="background:blue; color:white" |175/25
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!C
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|style="background:green"|150/50
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|style="background:green"|150/50
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|style="background:green"|150/50
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|style="background:blue; color:white" |175/25
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|style="background:blue; color:white" |175/25
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|style="background:blue; color:white" |175/25
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!D  
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|style="background:green"|150/50
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|style="background:green"|150/50
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|style="background:green"|150/50
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|style="background:blue; color:white" |175/25
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|style="background:blue; color:white" |175/25
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|style="background:blue; color:white" |175/25
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{| border="1"
|+'''96-wells plate'''
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====Counting and splitting cells====


==Results==
==Results==
*{{todo| WHAT?}}
*HT4 cells
** 286
** 249
** 203
** 193
*D12 hTERT cells (15.75×10<sup>4</sup> cells/mL)
** 20
** 12
** 15
** 16
** S<sup>+</sup> 12.62 mL + 2.38 mL cells (25.000 cells per well 24-wells plate (500 μL)
*D12 P28 of Mark hTERT cells (75.5×10<sup>4</sup> cells/mL)
**92
**67
**83
**60
**0.43 mL cells in 23 mL for 24-wells, 3.97 mL in 15 mL for 6 wells
* D9 (53.5×10<sup>4</sup> cells/mL)
** 52
**55
**64
**58
**0.61 mL in 13 mL and 5.35 mL in 15 mL
 
* ELISA {{todo|SEE DOSE EFFECT CURVE}}


==Conclusion==
==Conclusion==
*{{done}} Ow..
*{{done}} Ow..
===Related entries===
====Run 2: Ht31 Dose/Effect Curve & S<sup>0</sup> D9/D12====
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/19|Counting cells 19Feb2010]]
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/23|Putting to S<sup>0</sup> 23Feb2010]]
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/24|Stimulating cells 24Feb2010]]
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/25|Ending stimulation & Viability 25Feb2010]]
==Same action==
* [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/18| ELISA]]
* [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/12| Counting splitting cells]]
* [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/19| Counting splitting cells]]
==Attachment==
<html>
<a href="http://openwetware.org/wiki/Image:HTERT_Donor_12_ht31_160310.xls">
  <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">
</a>
</html> [[Media:HTERT_Donor_9_Ht31_010310_-_dose_effect_curve.xls| Excel Dose Effect curve D9]]
<br>
<html>
<a href="http://openwetware.org/wiki/Image:HTERT_Donor_12_ht31_160310.xls">
  <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">
</a>
</html> [[Media:HTERT_Donor_12_Ht31_010310_-_dose_effect_curve.xls| Excel Dose Effect curve D12]]




Revision as of 02:23, 23 March 2010

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

ELISA & Counting splitting cells <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

  • Short summary

Materials & Methods

Materials

Method

ELISA

Sample dilution ELISA (Dilution buffer (μL)/Sample (μL))
1 2 3 4 5 6
A 100/100 100/100 100/100 100/100 100/100 100/100
B 150/50 150/50 150/50 175/25 175/25 175/25
C 150/50 150/50 150/50 175/25 175/25 175/25
D 150/50 150/50 150/50 175/25 175/25 175/25

Counting and splitting cells

Results

  • HT4 cells
    • 286
    • 249
    • 203
    • 193
  • D12 hTERT cells (15.75×104 cells/mL)
    • 20
    • 12
    • 15
    • 16
    • S+ 12.62 mL + 2.38 mL cells (25.000 cells per well 24-wells plate (500 μL)
  • D12 P28 of Mark hTERT cells (75.5×104 cells/mL)
    • 92
    • 67
    • 83
    • 60
    • 0.43 mL cells in 23 mL for 24-wells, 3.97 mL in 15 mL for 6 wells
  • D9 (53.5×104 cells/mL)
    • 52
    • 55
    • 64
    • 58
    • 0.61 mL in 13 mL and 5.35 mL in 15 mL
  • ELISA SEE DOSE EFFECT CURVE

Conclusion

  • DONE Ow..

Related entries

Run 2: Ht31 Dose/Effect Curve & S0 D9/D12

Same action

Attachment

<html> <a href="http://openwetware.org/wiki/Image:HTERT_Donor_12_ht31_160310.xls"> 

  <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">

</a> </html> Excel Dose Effect curve D9
<html> <a href="http://openwetware.org/wiki/Image:HTERT_Donor_12_ht31_160310.xls"> 

  <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">

</a> </html> Excel Dose Effect curve D12



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