User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/19: Difference between revisions

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*{{done}} Ow..
*{{done}} Ow..
===Related entries===
===Related entries===
====Run 4: Ht31/S<sup>0</sup> D9/D12====
====Run 5: Ht31/S<sup>0</sup> D9/D12====
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/16|Splitting cells 16March2010]]
*[[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/16|Splitting cells 16March2010]]


Revision as of 14:36, 18 March 2010

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

  • Split cells
    • GIVE WOLTER 3×106 cells


  • D9
    • 1x 24-wells (25.000 cells/well (500 μL/well), 625000 cells/12.5 mL)
    • 2x Ø 10cm (750.000 cells/plate (10 mL/plate), 1500000 cells/20 mL)
    • 1x 6-wells (200.000 cells/well (2 mL/well), 1400000 cells/14 mL)
    • 8x Ø 15cm (1×106 cells/plate (15mL/plate), 3x 3×106 cells/45 mL)


  • D12
    • 1x 24-wells (25.000 cells/well (500 μL/well), 625.000 cells/12.5 mL)
    • 2x Ø 10cm (750.000 cells/plate (10 mL/plate), 1.500.000 cells/20 mL)
    • 1x 6-wells (200.000 cells/well (2 mL/well), 1.400.000 cells/14 mL)
    • 8x Ø 15cm (≤2×106 cells/plate (15 mL/plate), 3x ≤6×106 cells/45 mL)

Materials & Methods

Materials

  • 500 mL warm DMEM S+
  • 100 mL Warm PBS
  • 4x 15 mL tubes
  • 8x 50 mL tubes
  • 2x 24-wells plates
  • 4x Ø 10cm petridishes
  • 2x 6-wells plates
  • 16x Ø 15cm petridishes


  • 8 mL Trypin

Method

6-wells
# 1 2 3
A
B
24-wells
# 1 2 3 4 5 6
A
B
C
D
96-wells plate
# 01 02 03 04 05 06 07 08 09 10 11 12
A
B
C
D
E
F
G
H
I

Results

  • WHAT?

Conclusion

  • DONE Ow..

Related entries

Run 5: Ht31/S0 D9/D12


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