User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/23: Difference between revisions
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==Results== | ==Results== | ||
* | *Co-IP Blots of samples pulled down using PKA antibody coated beads, blots were treated with anti-AKAP antibody | ||
[[Image:230310_CoIP_AKAP_Gel_1.JPG|500px]]<br> | |||
Gel 1<br> | |||
[[Image:230310_CoIP_AKAP_Gel_2.JPG|500px]] | |||
Gel 2<br> | |||
# Only beads (30 μL) | |||
# Beads w. antibody (30 μL) | |||
# Beads w. antibody (30 μL) & lysis buffer (200 μL) | |||
# Beads w. antibody (30 μL) & sample (200 μL) | |||
A. [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/23|23Feb2010]] to [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/02/23|25Feb2010]]<br> | |||
B. [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/03|03March2010]] to [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/05|05March2010]]<br> | |||
C. [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/15|15March2010]] to [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/03/17|17March2010]] | |||
==Conclusion== | ==Conclusion== |
Revision as of 09:06, 23 March 2010
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Co-IP Part II, hTERT Stimulation & Western blot cont'd | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||
Summary
Materials & MethodsMaterials
MethodDetermination of protein concentration
Co-immunoprecipitation
hTERT stimulation
CTR = control, CSE = Cigarette Smoke extract, 8-pcpt = 8-pcpt-2'-o-me-camp Western blot, secondary Antibody
Preparing film
Notes
Results
A. 23Feb2010 to 25Feb2010 Conclusion
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