User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/05

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Co-IP day III, Stop stimulation hTERT, Split hTERT Main project page
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Summary

  • Short summary

Materials & Methods

Materials

Method

  • Co-IP day III
  • Stop stimulation D9/D12 24-wells induced yesterday (after 24 hours)
    • Alamar Blue
    • .. D9 ELISA samples got lost, D12 was put to 96-wells in freezer
  • Split cells D9/D12
    • 30.000 cells per well for 24-wells
    • 200.000 cells per well for 6 wells
    • 1 million per plate for 10cm petridish

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