User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/08
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Materials & Methods
Samples were loaded 40 μL, with 15 μL prestained protein marker in the first lane, according to the schedule below. Gell was run for 45-60 min @ 200 V until loading buffer had left the gel (+5 min). Proteins were transferred to membrane 1 h @ 100 V on ice.
Pierce Determination VASP 080310