User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/03/09

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hTERT stimulation @ splitting, Western blot contn'd Main project page
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Summary

Split D12 (p25) and D9 (p21) to 4 new petridishes (p+1)
24-wells D9 was put to S0
2x 6 wells (d12, d9) were refreshed with S+


Western blot
Primary antibody (1:1000 in 5% milk) (rabbit anti AKAP-79), 2 hours RT
wash 3x
secundary antibody (1:1000, 5% milk) (goat? anti-rabbit),1 hour RT
wash 3x
ECL, inc. 5 min. take pictures every 5 min, 6x
GAPDH 1:2000 ab for control protein amount


Stimulation D12 24 wells, S0/Ht31


Notes

  • 9.32 - 11.32 secondary antibody (1:1000 in 5% milk solution)

Results

  • WHAT?

PICTURE --> ANOUK

Conclusion

  • DONE Ow..

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