User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/05/18: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">RII Overlay & Stop stimulation ELISA</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Summary==
==Summary==
* RII overlay for blot made [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/09|09May2010]]
* RII overlay for blot made [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/09|09May2010]]
* Stop stimulation and collect samples for ELISA
* Stop stimulation and collect samples for ELISA
 
** Substrate buffer
==Materials & Methods==
===Materials===
*  
 
===Method===
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==Notes==
==Notes==
*Due to a missing filter the machine planned to use for viability staining a new machine had to be used and due to this more time had passed, plates were stored at 4 °C in the mean time.
* Fluorescence was not measurable anymore, absorbance was used instead.
==Results==
==Results==
*{{todo| WHAT?}}
*ALAMAR BLUE Viability staining see [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/05/19|19May2010]]


==Discussion==
==Discussion==
*{{done}} Ow..
* Alamar blue seems to work fine with only absorbance


==Related==
===Related entries===
===Same actions===
===Related topics===
==Attachment==
<html>
<a href="http://openwetware.org/wiki/Image:HTERT_Donor_12_ht31_160310.xls">
  <img src="http://openwetware.org/images/f/f8/Owwnotebook_icon.png" alt="" width="50" height="50">
</a>
</html> [[Media:FILE.xls| Excel TITLE]]
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|}
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__NOTOC__
__NOTOC__

Revision as of 08:21, 26 May 2010

<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>

RII Overlay & Stop stimulation ELISA <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Summary

  • RII overlay for blot made 09May2010
  • Stop stimulation and collect samples for ELISA
    • Substrate buffer

Notes

  • Due to a missing filter the machine planned to use for viability staining a new machine had to be used and due to this more time had passed, plates were stored at 4 °C in the mean time.
  • Fluorescence was not measurable anymore, absorbance was used instead.

Results

Discussion

  • Alamar blue seems to work fine with only absorbance


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