User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/05/19

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RII overlay cAMP precipitation <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

  • RII overlay
  • ELISA
    • Coating buffer for ELISA

Materials & Methods

Materials

Method

Notes

Results

  • Autoradiograph (ON exposure to phospho plate) of cAMP precipitation sizes shown are in kDa, calculated using non-Linear regression file for protein size on gel/membrane (found at starting page notebook)
  • Sample 5 is around the size of the RII unit (~55 kDa), the samples shown more to the righ (S0 samples) show the slope of the gel, where the band of 49 kDa should still be around the same hight as that of 53 kDa.

Discussion

  • DONE Ow..

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