User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/06/24: Difference between revisions

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(Autocreate 2010/06/24 Entry for User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization)
 
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==Notes==
==Notes==
==Results==
==Results==
*{{todo| WHAT?}}
*Immunoblot
[[Image:24062010_AKAP_immunoblot.png|500px]]
 
{| border="1"
|+'''Gel loading hTERT D9 + HEK293 see codes [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/06/21|21June2010]]'''
! align="center" style="background:#f0f0f0;"|#
! align="center" style="background:#f0f0f0;"|<u>1</u>
! align="center" style="background:#f0f0f0;"|<u>2</u>
! align="center" style="background:#f0f0f0;"|<u>3</u>
! align="center" style="background:#f0f0f0;"|<u>4</u>
! align="center" style="background:#f0f0f0;"|<u>5</u>
! align="center" style="background:#f0f0f0;"|<u>6</u>
! align="center" style="background:#f0f0f0;"|<u>7</u>
! align="center" style="background:#f0f0f0;"|<u>8</u>
! align="center" style="background:#f0f0f0;"|<u>9</u>
! align="center" style="background:#f0f0f0;"|<u>10</u>
|-
!Gel<br>cAMP prec.
| align="center"|X
| align="center"|HEK293<br>(20 μL)
| align="center"|[[Media:HiMark_protein_marker_-_Invitrogen.jpg|Marker]]<br> (8 μL)
| align="center"|C1<br>(20 μL)
| align="center"|C2<br>(20 μL)
| align="center"|C3<br>(20 μL)   
| align="center"|S1<br>(20 μL)
| align="center"|S2<br>(20 μL)
| align="center"|S3<br>(20 μL)
| align="center"|X
|-
|}


==Discussion==
==Discussion==

Revision as of 01:25, 25 June 2010

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Summary

  • Short summary

Materials & Methods

Materials

Method

6-wells
# 1 2 3
A
B
24-wells
# 1 2 3 4 5 6
A
B
C
D
96-wells plate
# 01 02 03 04 05 06 07 08 09 10 11 12
A
B
C
D
E
F
G
H
I

Notes

Results

  • Immunoblot

Gel loading hTERT D9 + HEK293 see codes 21June2010
# 1 2 3 4 5 6 7 8 9 10
Gel
cAMP prec. 		X HEK293
(20 μL) 		Marker
(8 μL) 		C1
(20 μL) 		C2
(20 μL) 		C3
(20 μL) 		S1
(20 μL) 		S2
(20 μL) 		S3
(20 μL) 		X

Discussion

  • DONE Ow..

Related

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