User:Wilfred J. Poppinga/Notebook/cAMP compartmentalization/2010/07/12: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">AKAP250 (scbt) immunoblot AKAP250 Co-IP</span> | ||
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==Summary== | ==Summary== | ||
* | * Washing the AKAP250 CO-IP Blot and adding secondary antibody | ||
* Adding AKAP250 antibody ZDE10030 animal 122 antibody (Eurogentech antibody service) to cAMP precipitation blot blocked in blocking buffer + 1 μL/mL Azide (20%stock) | |||
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==Materials & Methods== | ==Materials & Methods== | ||
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==Notes== | ==Notes== | ||
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==Results== | ==Results== | ||
* | *Immunoblot for AKAP250 on AKAP250 co-ip western blot | ||
[[Image:12072010_250co-ip_AKAP250_Blot.png|500px]] | |||
==Discussion== | ==Discussion== | ||
* | *Same as yesterday for the cAMP precipitation, can not really see any Gravin signals. Maybe very faint in S2 | ||
*Perhaps due to its aiming at the C-terminal the santa cruz C-16 antibody can not detect the protolytic fragment that might be seen on [[User:Wilfred_J._Poppinga/Notebook/cAMP_compartmentalization/2010/07/10|the ponceau of this membrane]] | |||
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==Related== | ==Related== | ||
===Related entries=== | ===Related entries=== |
Revision as of 05:40, 29 July 2010
<html><style type="text/css"> .todo { color: red } .done { color: green} </style></html>
AKAP250 (scbt) immunoblot AKAP250 Co-IP | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Summary
Results
Discussion
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